| Solution Information | help | |
| Enzyme: | Neurotensin receptor type 1 | |
| inhibitor: | BDBM61015 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | A. Brief Description of the Assay: This assay is to perform a 10 pt. dose-response on confirmed lead agonists that activate the Neurotensin receptor-1 (NTR1) pathway by detecting spots from beta-arrestin GFP in NTR1-U2OS Osteosarcoma Cells. B. Materials: Description Source Cat NTR1-U2OS osteosarcoma cells Dr. Lawrence Barak, Duke University N/A MEM medium Cellgro/Mediatech 15-010-CM Fetal Bovine Serum Hyclone SH30396.03 Penicillin Streptomycin solution Omega Scientific PS-20 L-Glutamine Mediatech 25-005-CL G418 (100 mg/mL) Invivogen ant-gn-1 Zeocin (100 mg/mL) Invitrogen R250-01 T225 Tissue Culture flasks Corning 431082 DPBS Cellgro 21-031CV Trypsin-EDTA 0.05% Invitrogen 25300 1536 well black clear bottom plate Aurora Biotechnology 29326 NTR1 peptide Sigma N6383 Paraformaldehyde Acros Organics 30528954 Hoechst 33342 (10 mg/mL) Invitrogen H3570 Aluminum Plate Seals E&K Scientific T592100 C. Plate Map: Positive (High) control (P) in columns 3, 4, 45 and 46, DMSO and 100 nM NTR1 Negat | |
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